alexa Multiplex real-time RT-PCR for detecting chikungunya virus and dengue virus.
Infectious Diseases

Infectious Diseases

Journal of Infectious Diseases & Therapy

Author(s): Pongsiri P, Praianantathavorn K, Theamboonlers A, Payungporn S, Poovorawan Y

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Abstract OBJECTIVE: To develop diagnostic test for detection chikungunya virus (CHIKV and Dengue virus (DENV) infection. METHODS: We have performed a rapid, accurate laboratory confirmative method to simultaneously detect, quantify and differentiate CHIKV and DENV infection by single-step multiplex real-time RT-PCR. RESULTS: The assay's sensitivity was 97.65\%, specificity was 92.59\% and accuracy was 95.82\% when compared to conventional RT-PCR. Additionally, there was no cross-reaction between CHIKV, DENV, Japanese encephalitis virus, hepatitis C, hepatitis A or hepatitis E virus. CONCLUSIONS: This rapid and reliable assay provides a means for simultaneous early diagnosis of CHIKV and DENV in a single-step reaction. Copyright © 2012 Hainan Medical College. Published by Elsevier B.V. All rights reserved. This article was published in Asian Pac J Trop Med and referenced in Journal of Infectious Diseases & Therapy

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