alexa Mutate Chlorella sp. by nuclear irradiation to fix high concentrations of CO2.
Chemical Engineering

Chemical Engineering

Journal of Bioprocessing & Biotechniques

Author(s): Cheng J, Huang Y, Feng J, Sun J, Zhou J, , Cheng J, Huang Y, Feng J, Sun J, Zhou J,

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Abstract To improve biomass productivity and CO2 fixation of microalgae under 15\% (v/v) CO2 of flue gas, Chlorella species were mutated by nuclear irradiation and domesticated with high concentrations of CO2. The biomass yield of Chlorella pyrenoidosa mutated using 500 Gy of (60)Co γ irradiation increased by 53.1\% (to 1.12 g L(-1)) under air bubbling. The mutants were domesticated with gradually increased high concentrations of CO2 [from 0.038\% (v/v) to 15\% (v/v)], which increased the biomass yield to 2.41 g L(-1). When light transmission and culture mixing in photo-bioreactors were enhanced at 15\% (v/v) CO2, the peak growth rate of the domesticated mutant (named Chlorella PY-ZU1) was increased to 0.68 g L(-1) d(-1). When the ratio of gas flow rate (L min(-1)) to 1L of microalgae culture was 0.011, the peak CO2 fixation rate and the efficiency of Chlorella PY-ZU1 were 1.54 g L(-1) d(-1) and 32.7\%, respectively. Copyright © 2013 Elsevier Ltd. All rights reserved. This article was published in Bioresour Technol and referenced in Journal of Bioprocessing & Biotechniques

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