alexa Mutational analysis of oncogenic AKT E17K mutation in common solid cancers and acute leukaemias.
Pathology

Pathology

Journal of Clinical & Experimental Pathology

Author(s): Kim MS, Jeong EG, Yoo NJ, Lee SH

Abstract Share this page

Abstract Mounting evidence indicates that alterations of AKT signalling play important roles in cancer development. An earlier study discovered an oncogenic AKT1 gene mutation (AKT1 E17K) in breast, colorectal and ovarian cancers. The aim of this study was to see whether the AKT1 E17K mutation is common in breast, colorectal, lung, gastric and hepatocellular carcinomas and acute leukaemias. We analysed the presence of the AKT1 E17K mutation in 731 cancer tissues by a single-strand conformation polymorphism assay. In addition, we analysed the corresponding sequences of AKT1 E17K in AKT2 and AKT3 genes. Overall, we detected the four AKT1 E17K mutations in the breast cancers (4/93; 4.3\%), but none in other cancers. There was no AKT2 or AKT3 mutation in the cancers. This study demonstrated that the AKT1 E17K mutation occurs in breast cancers at a low frequency, and that it is rare in other common cancers, including colorectal, lung, gastric and hepatocellular carcinomas and acute leukaemias. Despite the confirmed oncogenic function of the AKT1 E17K, the rare incidences of the mutation suggest that it may not play a crucial role in the development of the most common types of human cancers.
This article was published in Br J Cancer and referenced in Journal of Clinical & Experimental Pathology

Relevant Expert PPTs

Relevant Speaker PPTs

  • Ildiko Molnar
    The role of tissue-specific type 2 5’-deiodinase enzyme activities in Graves’ orbitopathy and systemic sclerosis: a new candidate in thyroid autoimmunity
    PDF Version
  • Abulkhair Beatti
    A new understanding of interferential current energy transfer in tissue
    PPT Version | PDF Version
  • Daisuke Ota
    The clinical outcome of reconstruction with tissue expander for breast cancer patients with mastectomy
    PPT Version | PDF Version
  • Mehmet Ozler
    “Mehmet Ozler-Gulhane-Military-Medical-Academy-Ankara-Turkey-Pinealectomy-Initiates-the-Oxidative-Stress-Response-in-Brain-Tissue-of-Rats-Subject-to-Abdominal-Surgery”
    PPT Version | PDF Version
  • Yosef Yarden
    Classically, the 3’untranslated region (3’UTR) is that region in eukaryotic protein-coding genes from the translation termination codon to the polyA signal. It is transcribed as an integral part of the mRNA encoded by the gene. However, there exists another kind of RNA, which consists of the 3’UTR alone, without all other elements in mRNA such as 5’UTR and coding region. The importance of independent 3’UTR RNA (referred as I3’UTR) was prompted by results of artificially introducing such RNA species into malignant mammalian cells. Since 1991, we found that the middle part of the 3’UTR of the human nuclear factor for interleukin-6 (NF-IL6) or C/EBP gene exerted tumor suppression effect in vivo. Our subsequent studies showed that transfection of C/EBP 3’UTR led to down-regulation of several genes favorable for malignancy and to up-regulation of some genes favorable for phenotypic reversion. Also, it was shown that the sequences near the termini of the C/EBP 3’UTR were important for its tumor suppression activity. Then, the C/EBP 3’UTR was found to directly inhibit the phosphorylation activity of protein kinase CPKC in SMMC-7721, a hepatocarcinoma cell line. Recently, an AU-rich region in the C/EBP 3’UTR was found also to be responsible for its tumor suppression. Recently we have also found evidence that the independent C/EBP 3’UTR RNA is actually exists in human tissues, such as fetal liver and heart, pregnant uterus, senescent fibroblasts etc. Through 1990’s to 2000’s, world scientists found several 3’UTR RNAs that functioned as artificial independent RNAs in cancer cells and resulted in tumor suppression. Interestingly, majority of genes for these RNAs have promoter-like structures in their 3’UTR regions, although the existence of their transcribed products as independent 3’UTR RNAs is still to be confirmed. Our studies indicate that the independent 3’UTR RNA is a novel non-coding RNA species whose function should be the regulation not of the expression of their original mRNA, but of some essential life activities of the cell as a whole.
    PPT Version | PDF Version
  • Kazuo Yano
    Regulatory approval for autologous human cells and tissue products in the United States, the European Union, and Japan
    PPT Version | PDF Version
  • William Lindsey
    Ultra-refined Follicular Unit Transplantation into scar tissue as an alternative to surgical scar revision in hair bearing scalp
    PPT Version | PDF Version
  • Simin Fazelipour
    “Simin Fazelipour-Islamic-Azad-University-Tehran-Medical-Sciences-Branch-Tehran-Iran-evaluation-of-histopathologic-and-histomorphometric-changes-of-adrenal-gland-tissue-following-consumption-of-Methylphenidate-in-male-mice”
    PPT Version | PDF Version
  • Momiao Xiong
    Integrative Image and RNA-seq Data Analysis
    PPT Version | PDF Version
  • Mehmet Saydam
    Quality-of- life, body image and cosmesis after single incision laparoscopic cholecystectomy versus laparoscopic cholecystectomy
    PPT Version | PDF Version
  • Jan O Gordeladze
    Key regulatory junctions stabilizing the osteoblast phenotype: Implications for cell and tissue engineering
    PPT Version | PDF Version
  • Marie Schneider
    Regenerative Potential of Outer Root Sheath Melanocytes in Tissue Grafts
    PPT Version | PDF Version
  • Mirette Hanna
    LOCAL INFLAMMATION IN BREAST TISSUE AND MAMMOGRAPHIC DENSITY AMONG PREMENOPAUSAL AND POSTMENOPAUSAL WOMEN
    PPT Version | PDF Version
  • Scott F Dye
    Current concepts on the etiology and safe treatment of the Patellofemoral pain enigma from a Tissue homeostasis perspective
    PPT Version | PDF Version
  • Monika Heidemarie Seltenhammer
    Detecting highly stabilized cumulative ~35-37kD isoforms of ΔFosB in postmortem human brain tissue samples of the nucleus accumbens (NAc) of chronic opioid abusers
    PPT Version | PDF Version

Recommended Conferences

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords