alexa n vitro Antioxidant and free radical scavenging activity of Macrotyloma uniflorum dal from Kumauni region
Chemical Engineering

Chemical Engineering

Journal of Chromatography & Separation Techniques

Author(s): Renu Singh, Manoj Kumar Singh, Lovy Raj Chandra, De epa Bhat, Manmeet Singh Arora

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Background & Aim: The present study was carried out to evaluate the in vitro antioxidant activities of Methanol extract of Dolichos biflorus dal ( (DME) ) commonly edible food from central Himalayans . Methods: This was achieved by screening of the plant extracts at vary ing concentrations (20-200μg/ml), using 2,2-dipheny l-1-picryl- hydrazyl (DPPH) radical scavenging activity, reduci ng power assay and hydrogen peroxide radical scaven ging activity. Results: Total phenol and flavonoid contents (92.10 ± 8.11 mg/ml GAE per 100 mg plant extract and 139.5 ± 55. 09 mg/ml QE equivalent per 100 mg plant extract) were found respectively. Scavenging effect of DME was 4 times greater than that of the synthetic antioxidant ascorbic aci d. Conclusion: Result also suggests a close relations in between to tal phenolic content and antioxidant activity, reducing power and radical scavenging effect on DPPH radica ls, which proves Dolichos biflorus has a potential source of useful natural antioxidants.

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This article was published in International Journal of Fundamental & Applied Sci ences and referenced in Journal of Chromatography & Separation Techniques

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