alexa Nanosecond pulse electric field (nanopulse): a novel non-ligand agonist for platelet activation.


Anatomy & Physiology: Current Research

Author(s): Zhang J, Blackmore PF, Hargrave BY, Xiao S, Beebe SJ,

Abstract Share this page

Abstract Nanosecond pulse stimulation of a variety of cells produces a wide range of physiological responses (e.g., apoptosis, stimulation of calcium (Ca2+) fluxes, changes in membrane potential). In this study, we investigated the effect of nanosecond pulses, which generate intense electric fields (nsPEFs), on human platelet aggregation, intracellular free Ca2+ ion concentration ([Ca2+]i) and platelet-derived growth factor release. When platelet rich plasma was pulsed with one 300ns pulse with an electric field of 30kV/cm, platelets aggregated and a platelet gel was produced. Platelet aggregation was observed with pulses as low as 7kV/cm with maximum effects seen with approximately 30kV/cm. The increases in intracellular Ca2+ release and Ca2+ influx were dose dependent on the electrical energy density and were maximally stimulated with approximately 30kV/cm. The increases in [Ca2+]i induced by nsPEF were similar to those seen with thapsigargin but not thrombin. We postulate that nsPEF caused Ca2+ to leak out of intracellular Ca2+ stores by a process involving the formation of nanopores in organelle membranes and also caused Ca2+ influx through plasma membrane nanopores. We conclude that nsPEFs dose-dependently cause platelets to rapidly aggregate, like other platelet agonists, and this is most likely initiated by the nsPEFs increasing [Ca2+]i, however by a different mechanism. This article was published in Arch Biochem Biophys and referenced in Anatomy & Physiology: Current Research

Relevant Expert PPTs

Relevant Speaker PPTs

  • Seema Narayan
    A comparative study of the link between real exchange rate of ASEAN countries and fossil fuel prices
    PDF Version
  • Navinchandra G. Ashar
    Comparative study of techno-economic evaluation of the production of liquid Sulphur Di Oxide
    PDF Version
  • Wei Gong
    Wei Gong-Huazhong-University-of-Science-and-Technology-China-Probabilistic-seismic-hazard-assessment-for-the-pseudo-negative-stiffness-control-of- a-steel-base-isolated-building-A-comparative-study-
    PPT Version | PDF Version
  • Abdullah Altin
    PPT Version | PDF Version
  • Nahla A El-Wakil
    Comparative study of enzymatic and chemical denaturation of wheat gluten and their cellulosic nanocomposites
    PPT Version | PDF Version
  • Krzysztof Wieczerzak
    A comparative transcriptome provides candidate genes for determination the cause of males infertility.
    PPT Version | PDF Version
  • Flavia Secco Tavares de Souza
    A Comparative Study Among Elective Conventional Surgery, Urgency/Emergency Conventional Approaching and Elective Videolaparoscopic Surgery on the Treatment of Hospitalized Patients at First Surgeric Clinic of Federal Hospital of Bonsucesso with a Diagnostic of Colorectal adenocarcinoma, between January 2010 and December 2012
    PPT Version | PDF Version
  • Rajendra K Ray
    Comparative Study And Improvement Of Shallow-Water Models
    PPT Version | PDF Version
  • Nisreen E. Mahmoud
    Parasitological and comparative pathological studies on monogenean infestation of cultured sea bream (Sparus aurata, Sparidae) in Egypt.
    PPT Version | PDF Version

Recommended Conferences

Relevant Topics

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version