alexa Nephrotoxic cell death by diclofenac and meloxicam
Geology & Earth Science

Geology & Earth Science

Journal of Geography & Natural Disasters

Author(s): Ng LE, Halliwell B, Wong KP

Abstract Share this page

The nephrotoxicity of diclofenac, a non-steroidal anti-inflammatory drug that inhibits both isoforms of cyclooxygenase (COX) has been reported to be fatal to vultures but this was not so with meloxicam which is COX-2 selective. Our study showed that diclofenac was more toxic than meloxicam to both the proximal tubular LLC-PK1 cells and the distal tubular Madin-Darby canine kidney type II (MDCKII) cells, and that LLC-PK1 cells were more susceptible. Exposure of MDCKII cells to meloxicam caused activation of caspase-9/-3 and release of cytochrome c. These observations together with a positive annexin V-FITC staining implicate an intrinsic mitochondrial cell death pathway by apoptosis. Diclofenac-treated MDCKII cells on the other hand showed extensive propidium iodide staining, suggestive of cell death by necrosis. The mode of cell death in LLC-PK1 cells was however less well-defined with positive annexin V-FITC staining but minimal increase in caspase-3 activity alluding to a caspase-independent pathway.

This article was published in Biochem Biophys Res Commun and referenced in Journal of Geography & Natural Disasters

Relevant Expert PPTs

Relevant Speaker PPTs

Recommended Conferences

  • 2nd World Congress on GIS and Remote Sensing
    July 20-21, 2017 Munich, Germany
  • 3rd World Congress on GIS and Remote Sensing
    September 20-21, 2017 Charlotte, North Carolina, USA

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords