alexa Nestin is induced by hypoxia and is attenuated by hyperoxia in Müller glial cells in the adult rat retina.
Genetics & Molecular Biology

Genetics & Molecular Biology

Journal of Stem Cell Research & Therapy

Author(s): Xue L, Ding P, Xiao L, Hu M, Hu Z

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Abstract This study investigated the reactive changes in Müller glial cells and astrocytes of the rat retinae, which had been subjected either to hypoxia or to hypoxia followed by hyperoxia treatments. Fifteen rats were used. Ten rats were exposed to 9\% O(2) for 2 h. Of these, five rats were killed at 24 h later; the remaining five rats were immediately exposed to 80\% O(2) for 2 h and then killed 24 h later. Double immunofluorescence was carried out between nestin and glutamine synthetase (GS) and between glial fibrilary acidic proteins (GFAP) and GS in normal and pathological retinae. Enhanced nestin expression was observed in reactive astrocytes following hypoxia treatment as revealed in whole mount sections. A novel finding was the induction of nestin expression in Müller glial cells. Remarkably, the nestin immunostaining was downregulated to levels comparable to those of the normal rats with immediate hyperoxia treatment. Induced nestin expression by hypoxia colabelled with GFAP in astrocytes, however, remained unaffected after hyperoxia treatment. The induced expression of nestin in Müller glial cells and astrocytes in hypoxia and differential downregulation after hyperoxia treatment suggest a structural plasticity of the cytoskeletal framework of these cells. The differential response after hyperoxia treatment may be related to the functional states of the cells. © 2011 The Authors. International Journal of Experimental Pathology © 2011 International Journal of Experimental Pathology.
This article was published in Int J Exp Pathol and referenced in Journal of Stem Cell Research & Therapy

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