alexa New Method for Quantitative Determination of Uranic Acids
Biochemistry

Biochemistry

Enzyme Engineering

Author(s): ELLY BLUMENKRANTZ, GUSTAV ASBOEHANSEN

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Hexosamine and uranic acid are the components of the repeating unit of all acid mucopolysaccharides (glycosaminoglycans) with the exception of keratosulfate, in which galactose replaces the uranic acid moiety. Uranic acid is widely determined as representative of acid mucopolysaccharides in biological substances. However, the assays mainly used for quantitative determination of uranic acids are not suf- ficiently accurate, insofar as hexoses (1,2) or pentoses (3) interfere with their specificity. The need for a rapid, sensitive, and specific method for quantitative assay of uranic acids and acid mucopolysaccharides stimu- lated the elaboration of a new method based upon the appearance of a chromogen when uranic acid heated to 100°C in concentrated sulfuric acid/tetraborate is treated with meta-hydroxydiphenyl

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This article was published in ANALYTICAL BIOCHEMISTRY and referenced in Enzyme Engineering

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