alexa New validated method for piracetam HPLC determination in human plasma.
Pharmaceutical Sciences

Pharmaceutical Sciences

Journal of Applied Pharmacy

Author(s): Curticapean A, Imre S

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Abstract The new method for HPLC determination of piracetam in human plasma was developed and validated by a new approach. The simple determination by UV detection was performed on supernatant, obtained from plasma, after proteins precipitation with perchloric acid. The chromatographic separation of piracetam under a gradient elution was achieved at room temperature with a RP-18 LiChroSpher 100 column and aqueous mobile phase containing acetonitrile and methanol. The quantitative determination of piracetam was performed at 200 nm with a lower limit of quantification LLQ=2 microg/ml. For this limit, the calculated values of the coefficient of variation and difference between mean and the nominal concentration are CV\%=9.7 and bias\%=0.9 for the intra-day assay, and CV\%=19.1 and bias\%=-7.45 for the between-days assay. For precision, the range was CV\%=1.8/11.6 in the intra-day and between-days assay, and for accuracy, the range was bias\%=2.3/14.9 in the intra-day and between-days assay. In addition, the stability of piracetam in different conditions was verified. Piracetam proved to be stable in plasma during 4 weeks at -20 degrees C and for 36 h at 20 degrees C in the supernatant after protein precipitation. The new proposed method was used for a bioequivalence study of two medicines containing 800 mg piracetam. This article was published in J Biochem Biophys Methods and referenced in Journal of Applied Pharmacy

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