Author(s): Ramachandran N, Raphael JV, Hainsworth E, Demirkan G, Fuentes MG,
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Abstract We developed a high-density self-assembling protein microarray, based on the nucleic acid programmable protein array (NAPPA) concept, to display thousands of proteins that are produced and captured in situ from immobilized cDNA templates. We arrayed up to 1,000 unique human cDNAs and obtained high yields of protein expression and capture with minimal variation and good reproducibility. This method will enable various experimental approaches to study protein function in high throughput.
This article was published in Nat Methods
and referenced in Journal of Clinical & Cellular Immunology