Author(s): Ostle AG, Holt JG
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Abstract Poly-beta-hydroxybutyrate granules exhibited a strong orange fluorescence when stained with Nile blue A. Heat-fixed cells were treated with 1\% Nile blue A for 10 min and were observed at an excitation wavelength of 460 nm. Glycogen and polyphosphate did not stain. Nile blue A appears to be a more specific stain for poly-beta-hydroxybutyrate than Sudan black B.
This article was published in Appl Environ Microbiol
and referenced in Journal of Antivirals & Antiretrovirals