alexa Non-destructive quantitation of spermine in human prostate tissue samples using HRMAS 1H NMR spectroscopy at 9.4 T.
Microbiology

Microbiology

Journal of Antivirals & Antiretrovirals

Author(s): Cheng LL, Wu C, Smith MR, Gonzalez RG

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Abstract We present the results of a study of human prostate specimens evaluated by high resolution magic angle spinning (1)H nuclear magnetic resonance (NMR) spectroscopy at 400 MHz (9.4 T) and by quantitative histopathology. We demonstrate that NMR and pathology data can be obtained from the same intact specimens, and report for the first time a linear correlation between the NMR measured concentration of spermine, a proposed endogenous inhibitor to prostate cancer growth, and the volume percentage of normal prostatic epithelial cells as quantified by histopathology. Our results show that NMR may serve as a critical tool for the investigation of the inhibitory mechanism of spermine in human subjects.
This article was published in FEBS Lett and referenced in Journal of Antivirals & Antiretrovirals

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