Author(s): Farthing D, Sica DA, Fakhry I, Gehr TW
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Abstract A novel solid-phase on-line elution HPLC method employing fluorescence detection to measure metolazone in plasma and whole blood has been developed. The method is sensitive and selective for metolazone and linear over a dynamic range of 1-50 ng/ml with a sample requirement of 250 microliters. The limit of quantitation for the method is 1 ng/ml and combined intra- and inter-day accuracy and precision had an error and coefficient of variation of 2.9 and 5.5\%, respectively.
This article was published in J Chromatogr B Biomed Appl
and referenced in Journal of Bioanalysis & Biomedicine