alexa Nucleotide sequence of the hepatitis B virus genome (subtype ayw) cloned in E. coli.
Infectious Diseases

Infectious Diseases

Journal of Infectious Diseases and Diagnosis

Author(s): Galibert F, Mandart E, Fitoussi F, Tiollais P, Charnay P

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Abstract The complete nucleotide sequence of hepatitis B virus genome (subtype ayw) cloned in Escherichia coli has been determined using the Maxam and Gilbert method and the dideoxynucleotide method. This sequence is 3,182 nucleotides long. Location of the nonsense codons shows that the coding capacity of the L chain is larger than the coding capacity of the S chain. Eight open regions, able to code for polypeptide chains larger than 100 amino acids, have been located. Region 6, which is the largest, covers more than 80\% of the genome. The gene S which codes for polypeptide I of the Hbs Ag and was previously located between coordinates 95.1 and 73.6 is contained in region 7.
This article was published in Nature and referenced in Journal of Infectious Diseases and Diagnosis

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