Author(s): AlModhefer AJ, Bradbury MW, Simons TJ, AlModhefer AJ, Bradbury MW, Simons TJ
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Abstract 1. The binding of lead to human blood serum, and components of serum, was studied by titration with the addition of Pb(NO3)2 solution, monitoring the free Pb2+ concentration with a Pb2+ electrode, and by equilibrium dialysis. 2. In fresh serum, about 4999 out of 5000 parts of added lead were bound. This suggests that the free Pb2+ concentration is around 1/5000th of the total lead concentration in the serum of normal subjects, i.e. about 1 x 10(-12) mol/l. 3. About 60\% of the binding of lead in serum is abolished by standing in air, by dialysis or by treatment with N-ethylmaleimide. This appears to be due to the presence of thiol compounds, mainly cysteine. The remaining 40\% appears to be due to protein, mainly albumin.
This article was published in Clin Sci (Lond)
and referenced in Journal of Drug Metabolism & Toxicology