Author(s): Bentz EK, Kenning M, Schneeberger C, Kolbus A, Huber JC,
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Abstract BACKGROUND: The stem cell marker Octamer-4 (OCT-4) is expressed in human endometrium. Menstrual cycle-dependency of OCT-4 expression has not been investigated to date. METHODS: In a prospective, single center cohort study of 98 women undergoing hysteroscopy during the follicular (n = 49) and the luteal (n = 40) phases of the menstrual cycle, we obtained endometrial samples. Specimens were investigated for OCT-4 expression on the mRNA and protein levels using reverse transcriptase polymerase chain reaction (RT-PCR) and immunohistochemistry. Expression of OCT-4 was correlated to menstrual cycle phase. RESULTS: Of 89 women sampled, 49 were in the follicular phase and 40 were in the luteal phase. OCT-4 mRNA was detected in all samples. Increased OCT-4 mRNA levels in the follicular and luteal phases was found in 35/49 (71\%) and 27/40 (68\%) of women, respectively (p = 0.9). Increased expression of OCT-4 protein was identified in 56/89 (63\%) samples. Increased expression of OCT-4 protein in the follicular and luteal phases was found in 33/49 (67\%) and 23/40 (58\%) of women, respectively (p = 0.5). CONCLUSIONS: On the mRNA and protein levels, OCT-4 is not differentially expressed during the menstrual cycle. Endometrial OCT-4 is not involved in or modulated by hormone-induced cyclical changes of the endometrium.
This article was published in Reprod Biol Endocrinol
and referenced in Reproductive System & Sexual Disorders: Current Research