alexa On the accuracy of instantaneous gas exchange rates, energy expenditure and respiratory quotient calculations obtained from indirect whole room calorimetry.
Diabetes & Endocrinology

Diabetes & Endocrinology

Diabetes Case Reports

Author(s): Gribok A, Hoyt R, Buller M, Rumpler W

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Abstract This paper analyzes the accuracy of metabolic rate calculations performed in the whole room indirect calorimeter using the molar balance equations. The equations are treated from the point of view of cause-effect relationship where the gaseous exchange rates representing the unknown causes need to be inferred from a known, noisy effect-gaseous concentrations. Two methods of such inference are analyzed. The first method is based on the previously published regularized deconvolution of the molar balance equation and the second one, proposed in this paper, relies on regularized differentiation of gaseous concentrations. It is found that both methods produce similar results for the absolute values of metabolic variables and their accuracy. The uncertainty for O2 consumption rate is found to be 7\% and for CO2 production--3.2\%. The uncertainties in gaseous exchange rates do not depend on the absolute values of O2 consumption and CO2 production. In contrast, the absolute uncertainty in respiratory quotient is a function of the gaseous exchange rates and varies from 9.4\% during the night to 2.3\% during moderate exercise. The uncertainty in energy expenditure was found to be 5.9\% and independent of the level of gaseous exchange. For both methods, closed form analytical formulas for confidence intervals are provided allowing quantification of uncertainty for four major metabolic variables in real world studies. This article was published in Physiol Meas and referenced in Diabetes Case Reports

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