Author(s): Saris NE
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Abstract Rat liver mitochondria are able to temporarily lower the steady state concentration of external Ca2+ after having accumulated a pulse of added Ca2+. This could be due to inhibition of efflux or/and stimulation of influx of Ca2+. This question has been addressed in mitochondria respiring on succinate +/- malonate. In the presence of malonate the depression of the membrane potential during Ca2+ uptake is more extensive and the rate of Ca2+ uptake slower. There were no discernible differences in the rates of efflux either after inhibition of the calcium uniporter by Ruthenium Red or by studying efflux of preloaded 45Ca-labeled Ca2+. The efflux was not changed by diltiazem or cyclosporin A to inhibit Ca2+ exchange on the Ca2+/nNA+ antiporter or efflux through the permeability transition pore. It is concluded that the rebounding is due mainly to stimulation of the calcium uniporter.
This article was published in Biol Chem
and referenced in Biochemistry & Analytical Biochemistry