alexa One step ahead: role of filopodia in adhesion formation during cell migration of keratinocytes.
Genetics & Molecular Biology

Genetics & Molecular Biology

Journal of Tissue Science & Engineering

Author(s): Schfer C, Borm B, Born S, Mhl C, Eibl EM,

Abstract Share this page

Abstract Cell adhesion is an essential prerequisite for cell function and movement. It depends strongly on focal adhesion complexes connecting the extracellular matrix to the actin cytoskeleton. Especially in moving cells focal adhesions are highly dynamic and believed to be formed closely behind the leading edge. Filopodia were thought to act mainly as guiding cues using their tip complexes for elongation. Here we show for keratinocytes a strong dependence of lamellipodial adhesion sites on filopodia. Upon stable contact of the VASP-containing tip spot to the substrate, a filopodial focal complex (filopodial FX) is formed right behind along the filopodia axis. These filopodial FXs are fully assembled, yet small adhesions containing all adhesion markers tested. Filopodial FXs when reached by the lamellipodium are just increased in size resulting in classical focal adhesions. At the same time most filopodia regain their elongation ability. Blocking filopodia inhibits development of new focal adhesions in the lamellipodium, while focal adhesion maturation in terms of vinculin exchange dynamics remains active. Our data therefore argue for a strong spatial and temporal dependence of focal adhesions on filopodial focal complexes in keratinocytes with filopodia not permanently initiated via new clustering of actin filaments to induce elongation. This article was published in Exp Cell Res and referenced in Journal of Tissue Science & Engineering

Relevant Expert PPTs

Relevant Speaker PPTs

Recommended Conferences

  • 6th International Conference on Tissue Engineering and Regenerative Medicine
    August 23-24 , 2017 San Francisco, California ,USA
  • 8th International Conference on Tissue Science and Regenerative Medicine
    September 11-12, 2017 Singapore City, singapore
  • 9th Annual Conference on Stem Cell and Regenerative Medicine
    Sep 25-26, 2017 Berlin, Germany
  • 10th World Congress on Stem Cell and Biobanking
    October 23-24, 2017 Osaka, Japan

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords