alexa One-step purification and kinetic properties of the recombinant L-asparaginase from Erwinia carotovora.
Microbiology

Microbiology

Journal of Microbial & Biochemical Technology

Author(s): Krasotkina J, Borisova AA, Gervaziev YV, Sokolov NN

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Abstract ECAR-LANS, the recombinant L-asparaginase from Erwinia carotovora, is a prospective therapeutic enzyme for leukaemia treatment. An efficient and economical scheme was developed for the purification, cloning and expression in Eschericha coli of ECAR-LANS. More than 90\% purity, complemented with 72\% active enzyme recovery, was achieved with a single chromatographic purification step. The activity of purified L-asparaginase was 630 i.u./mg. The ECAR-LANS K (m) value was 98x10(-6) M for the main physiological substrate L-Asn and 3400x10(-6) M for L-Gln. ECAR-LANS was found to have low relative glutaminase activity (1.2\%) at physiological concentrations of L-Asn and L-Gln in blood. Kinetic studies of ECAR-LANS showed that the recombinant asparaginase combined the main advantages of Erw. chrysanthemi and E. coli L-asparaginases II, currently used in the treatment of acute lymphoblastic leukaemia. This article was published in Biotechnol Appl Biochem and referenced in Journal of Microbial & Biochemical Technology

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