alexa Online LC-MS-MS process monitoring for optimization of biological treatment of wastewater containing azo dye concentrates.
Environmental Sciences

Environmental Sciences

Journal of Bioremediation & Biodegradation

Author(s): Rehorek A, Plum A

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Abstract A biological high-performance treatment process comprising two 40-L reactor compartments has been developed for purification and decoloration of concentrated textile wastewater containing up to 15 g L(-1) reactive dyestuff. The decoloration rate of 95\% meets the requirements of German legislation for textile wastewater treatment. Successful process development and optimization was achieved by use of high-performance liquid chromatography, with diode-array and electrospray tandem mass spectrometric (LC-ESI-MS-MS) detection, coupled with inline microfiltration membrane-sampling devices, applied online to bioreactors as a process analytical tool for the first time. The optimum process performance was found by correlation of dye and decoloration product-specific concentrations with summary properties such as redox potential and dissolved oxygen content. Details of the degradation and decoloration mechanism for the azo dye reactive black 5 was revealed by using mass spectrometry for structure elucidation. This article was published in Anal Bioanal Chem and referenced in Journal of Bioremediation & Biodegradation

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