alexa Optimization of annealing temperature to reduce bias caused by a primer mismatch in multitemplate PCR.
General Science

General Science

Journal of Forensic Research

Author(s): Ishii K, Fukui M, Ishii K, Fukui M

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Abstract To reduce PCR bias derived from a primer mismatch, the effect of the annealing temperature on the product ratio was investigated by denaturing gradient gel electrophoresis analysis of PCR products from a mixture of perfect-match and one-mismatch templates. These templates were generated by PCR from Pediococcus acidilactici for one mismatch and Micrococcus luteus for the perfect match. PCRs showed that the bias was reduced at lower temperatures. An environmental sample was also examined.
This article was published in Appl Environ Microbiol and referenced in Journal of Forensic Research

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