Author(s): Kavya V, Padmavathi T
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Abstract The objectives of the present study were isolation, identification and characterization of xylanase producing fungi, optimization of medium composition and cultural conditions for xylanase enzyme production using cheaper sources. The fungal strains were isolated from garden soil by serial dilution technique and Aspergillus niger was identified and isolated in pure form. In conformation screening by congo red test, based on the reddish zone of enzyme activity formation in oat spelt xylan agar plates, A. niger was selected and optimized for xylanase enzyme production in solid state fermentation using cheaper sources like wheat bran, rice bran, soya bran, ragi bran and dust. Maximum enzyme activity was observed in wheat bran (9.87 U/ml). The use of wheat bran as a major carbon source is particularly valuable because oat spelt xylan or birch wood xylan are more expensive. The effects of time course, incubation substrate, inoculum size, moisturizing agent, moisture content, temperature and volume of fermentation medium on the production of xylanase were studied. The maximum xylanase production (12.65 U/ml) was observed at optimized condition, incubation temperature of 28 degrees C after 6 days of incubation period while minimum production (9.38 U/ml) at unoptimized condition. The maximum production of enzyme was found to be in wheat bran when the volume of fermentation medium was kept as 10 g/250 ml conical flasks, with mineral solution as moisturizing agent and moisture ratio 1:0.7. Thus the present study proved that the fungal strain A. niger used is highly potential and useful for xylanase production.
This article was published in Pol J Microbiol
and referenced in Journal of Biodiversity, Bioprospecting and Development