alexa Optimization of Inocula Conditions for Enhanced Biosurfactant Production by Bacillus subtilis SPB1, in Submerged Culture, Using Box-Behnken Design.
Environmental Sciences

Environmental Sciences

Journal of Petroleum & Environmental Biotechnology

Author(s): Mnif I, , EllouzeChaabouni S, Ghribi D,

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Abstract The inoculum age and density can influence considerably the production yield and cost of the fermentation process. Some literature studies report the use of two-stage inocula to enhance metabolite production. In the present study, optimization studies were done in order to define the best inocula conditions supporting a maximum biosurfactant production by Bacillus subtilis SPB1. Hence, by adjusting the levels of the two-stage inocula strategy, lipopeptide production was effectively enhanced to almost 3.4 g/l as estimated gravimetrically. The new defined parameters were as follows; a first inoculum age of 23 h followed by a second inoculum age and size of 4 h and 0.01, respectively. Thereby, we note an improved production as compared to the production yield described under non-optimized inocula conditions reported in our previous work. This article was published in Probiotics Antimicrob Proteins and referenced in Journal of Petroleum & Environmental Biotechnology

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