Author(s): Ramnani P, Gupta R
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Abstract A 3.5-fold increase in keratinase production by Bacillus licheniformis RG1 was achieved by using statistical methods involving Plackett-Burman design and response surface methodology. Eight variables were screened using Plackett-Burman design. Of these, glucose, peptone and glutathione were found to affect the response signal positively, whereas CaCl(2) had a negative effect. Further interaction of these factors, along with phosphate and incubation time, was studied using response surface methodology. An optimum keratinase production of 1295 units/mg dry weight was obtained with the following medium composition: 1\% glucose, 1\% peptone, 1\% phosphate, 0.05\% glutathione, 0.5\% feather and 2\% inoculum under shaking at 250 rev./min with an incubation period of 72 h at 37 degrees C. Keratinase production was found to be a function of biomass and maximum production occurred during the stationary phase.
This article was published in Biotechnol Appl Biochem
and referenced in Journal of Bioprocessing & Biotechniques