alexa Optimization of medium for enhancement of ε-poly-L-lysine production by Streptomyces sp. M-Z18 with glycerol as carbon source.
Environmental Sciences

Environmental Sciences

Journal of Bioremediation & Biodegradation

Author(s): Chen X, Tang L, Li S, Liao L, Zhang J,

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Abstract The goal of this study was to increase ε-Poly-L-Lysine (ε-PL) production by Streptomyces sp. M-Z18 through optimization of the culture medium. Glycerol, (NH4)2SO4 and K2HPO4 were the key factors in the fermentation according to the results obtained from the Plackett-Burman experimental design. The optimal concentration of the three factors was further investigated according to the central composite design and determined to be 59.48, 4.43 and -0.49 g/L, respectively. Under optimal conditions, a ε-PL concentration of 2.27±0.15 g/L was obtained. Compared with glucose, fermentation of glycerol afforded 2.8-fold and 6.3-fold the ε-PL titer and yield, respectively. Therefore, glycerol may be an efficient carbon source for ε-PL production by Streptomyces sp. M-Z18. Copyright © 2010 Elsevier Ltd. All rights reserved. This article was published in Bioresour Technol and referenced in Journal of Bioremediation & Biodegradation

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