alexa Optimization of procedures for isolation of mycobacteria from soil and water samples obtained in northern India.


Mycobacterial Diseases

Author(s): Parashar D, Chauhan DS, Sharma VD, Chauhan A, Chauhan SV,

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Abstract For isolation of environmental mycobacteria, a decontamination procedure has been standardized by which treatment with 3\% sodium dodecyl sulfate plus 4\% NaOH (15 and 30 min for rapid and slow growers, respectively) is followed by incubation with 2\% cetrimide (5 and 15 min for fast- and slow-growing mycobacteria, respectively); this procedure was found to completely eliminate contamination with other organisms and resulted in the isolation of only mycobacteria.
This article was published in Appl Environ Microbiol and referenced in Mycobacterial Diseases

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