alexa Ovine interleukin-12: analysis of biologic function and species comparison.
General Science

General Science

Journal of Biotechnology & Biomaterials

Author(s): De Rose R, Scheerlinck JP, Casey G, Wood PR, Tennent JM,

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Abstract Interleukin-12 (IL-12) is a heterodimeric cytokine produced mainly by phagocytic and antigen-presenting cells (APC). The cDNA encoding the ovine IL-12 (OvIL-12) subunits, p40 and p35, were generated from concanavalin A (ConA)-stimulated peripheral blood mononuclear cells (PBMC). The ovine genes encoded proteins that had the highest amino acid identity to caprine p40 (99\% amino acid identity) and p35 (97\% amino acid identity) and also displayed a high degree of identity with human p40 (84\%) and p35 (79\%) homologs. To ensure the equal expression of both subunits, we used the self-cleaving properties of the 2A oligopeptide from foot-and-mouth disease virus (FMDV) to express IL-12 as a single, long open reading frame (ORF) encoding p402Ap35. Using an in vitro transcription/translation system, we demonstrated that this 2A oligopeptide mediated cleavage of the p402Ap35 into p402A and p35, in a manner similar to the processing of the FMDV polypeptide. Moreover, when expressed in COSm6 cells, this self-processing polypeptide encoded a functional heterodimer, which elicited biologic activities associated with IL-12 in other species. This article was published in J Interferon Cytokine Res and referenced in Journal of Biotechnology & Biomaterials

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