alexa Partial sequencing and mapping of clones from two maize cDNA libraries.


Journal of Antivirals & Antiretrovirals

Author(s): Shen B, Carneiro N, TorresJerez I, Stevenson B, McCreery T,

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Abstract As one component of a maize genome project, we report the analysis of a number of randomly selected cDNAs, by a combination of measuring mRNA expression, 'single-pass' sequencing (SPS), and genome mapping. Etiolated seedling (490) and membrane-free polysomal endosperm cDNA clones (576) were evaluated for their transcription levels by hybridizing with a probe prepared from total mRNA and categorized as corresponding to abundantly or rarely expressed mRNAs and as either constitutive or tissue-specific. A total 313 clones from the two libraries were submitted to 'single-pass' sequencing from the presumed 5' end of the mRNA and the nucleotide sequence compared with the GenBank database. About 61\% of the clones showed no significant similarities within GenBank, 14\% of the clones exhibited a high degree of similarity, while the remaining 25\% exhibited a lesser degree of similarity. The chromosomal location of more than 300 clones was determined by RFLP mapping using standard populations. The results demonstrate that a combination of analyses provides synergistic information in eventually deducing the actual function of these types of clones.
This article was published in Plant Mol Biol and referenced in Journal of Antivirals & Antiretrovirals

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