Author(s): Battaglia A, Schweighardt AJ, Wallace MM
Abstract Share this page
Abstract Low concentrations of microbial pathogens in pure and mixed samples were detected using a bead-based, liquid array technology. A 20-bp sequence in the 23S rRNA gene, rrl, was amplified in four microorganisms: Bacillus cereus, Escherichia coli, Salmonella enterica and Staphylococcus aureus. PCR products were positively identified with the Luminex(®) 100™ system. The system could detect very low amounts of DNA and the instrument response was proportional to the input concentration. The lower limit of detection (LLD) was determined to be 0.5 ng for B. cereus and E. coli and 2 ng for S. enterica. The LLD for S. aureus was not determined as the instrument response was still above the threshold when quantities of DNA as low as 0.25 ng were used. The platform positively identified organisms present in mixed samples even when the minor component was overshadowed by a 10-fold excess of the major component. © 2011 American Academy of Forensic Sciences.
This article was published in J Forensic Sci
and referenced in Journal of Analytical & Bioanalytical Techniques