alexa Pathway of C6-NBD-Ceramide on the host cell infected with Toxoplasma gondii.
Pathology

Pathology

Journal of Clinical & Experimental Pathology

Author(s): de Melo EJ, de Souza W

Abstract Share this page

Fluorescence microscopy, with dyes analog of ceramide, and transmission electron microscopy, were used to analyze lipid traffic during interaction of Toxoplasma gondii with host cells. It is C6-NBD-Ceramide (C6-NBD-Cer), a fluorescent analog of ceramide, stained the Golgi complex where was metabolized into fluorescent sphingolipid and glucosylceramide, and translocated via the Golgi complex to the plasma membrane of living cells. In uninfected cells, C6-NBD-Cer initially concentrated at the perinuclear region, and after its fluorescent products were present in the cytoplasm and the plasma membrane. In infected cells, the probe initially is stained the Golgi complex. After 4 hours incubation with C6-NBD-Cer, the parasites within the parasitophorous vacuole began to be stained, and at 5 hours incubation, the parasites are completely fluorescent. The Golgi complex, as revealed by fluorescent probe and electron microscopy, maintained its perinuclear position throughout the evolution of intracellular parasitism. These results suggested that the intracellular parasite used the lipid pathway of the host cell.

This article was published in Cell Struct Funct. and referenced in Journal of Clinical & Experimental Pathology

Relevant Expert PPTs

Relevant Speaker PPTs

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords