alexa Penetrating corneal transplantation in the inbred rat: a new model.
Molecular Biology

Molecular Biology

Journal of Cell Science & Therapy

Author(s): Williams KA, Coster DJ

Abstract Share this page

Abstract A model of orthotopic penetrating keratoplasty has been developed in the inbred rat using both avascular and prevascularized recipient beds. The surgical procedure is conventional and can be achieved with standard instrumentation. Isografts into avascular recipient beds (Fisher 344 into Fisher 344 strain combination) were successful and survived indefinitely with excellent corneal function judged either visually by clarity and lack of oedema, or histologically at autopsy. Allografts into avascular beds (DA into Fisher 344 strain combination) became cloudy and oedematous at a median of day 12 postgraft; 43\% spontaneously recovered clarity while the remaining 57\% remained opaque or became scarred. Penetrating grafts also were performed in eyes prevascularized by the placement of sutures approximately 3 weeks prior to transplantation. Most isografts into prevascularized and inflamed beds underwent a transient episode of oedema, which quickly resolved and was felt to result from postsurgical inflammation. All allografts into prevascularized beds became oedematous and cloudy; 76\% went on to fail completely, while 24\% cleared without treatment. End-point histology showed normal graft morphology in the isografts; failed allografts showed a picture consistent with immunologic rejection. The model, which allows corneal transplantation to be performed against a constant histocompatibility barrier, may be useful in studies of rejection.
This article was published in Invest Ophthalmol Vis Sci and referenced in Journal of Cell Science & Therapy

Relevant Expert PPTs

Relevant Speaker PPTs

Recommended Conferences

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords