Author(s): Tatsuma T, Watanabe T
Abstract Share this page
Abstract A peroxidase model electrode was devised for reagentless sensing of hydrogen peroxide (H2O2). A small model molecule, which mimics the vicinity of the reaction center of a redox enzyme, can communicate electrochemically with an electrode. Heme nonapeptide (MW congruent to 1600) having peroxidase activity was adopted as a peroxidase model compound and was covalently immobilized on a tin oxide (SnO2) electrode as a roughly monomolecular layer. The modified electrode thus obtained responded to H2O2 at concentrations down to 10(-6) M without electron mediator or promoter, at a mild potential of +150 or +300 mV vs Ag/AgCl. In a batch system, the response reached a steady state in a few seconds. Measurements were possible also in a flow system with an assay time of 0.5-1.0 min/sample. The steady-state response of the electrode was kinetically analyzed.
This article was published in Anal Chem
and referenced in Journal of Microbial & Biochemical Technology
- X. Gomez
Feasibility of biofuels production: combining H2-CH4 and lipid production from food wastes using mixed anaerobic microflora
PPT Version | PDF Version