alexa Persistence of tick-borne encephalitis virus in monkeys. II. Effectiveness of methods used for virus detection.
General Science

General Science

Journal of Bioterrorism & Biodefense

Author(s): Pogodina VV, Malenko GV, Fokina GI, Levina LS, Koreshkova GV,

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Abstract Fifty monkeys (Macaca rhesus) inoculated with tick-borne encephalitis (TBE)virus intracerebrally or subcutaneously were examined. The efficiencies of different virus detection methods at 90 to 783 days after inoculation were: explantation of organs - 41.2\%; co-cultivation of trypsinized organ cells and indicator cells in the presence of 5-bromo-2-iododeoxyuridine with detection of the virus-specific antigen by immunofluorescence - 43.7\%, detection of infectious virus and complement-fixing antigen - 13.6\%; immunofluorescence examination of organ impression smears - 29.3\%; and examination of organ homogenates with detection of virus pathogenic for mice - 1.7\%. the latter method was the least sensitive for the detection of persisting TBE virus, in spite of that in the first 3 weeks of infection its efficacy was 88.1\% in examinations of clinically ill monkeys and 23.8\% in the inapparent form of the infection.
This article was published in Acta Virol and referenced in Journal of Bioterrorism & Biodefense

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