Author(s): Yang XL, Fan CH, Zhu HS, Yang XL, Fan CH, Zhu HS, Yang XL, Fan CH, Zhu HS, Yang XL, Fan CH, Zhu HS
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Abstract The biological activities of fullerenes have attracted extensive attention in recent years. The aim of this paper is to study the relation of the photo-induced cytotoxicity of fullerene derivatives to their chemical structures as well as the possible cellular mechanism involved in the photocytotoxicity. Three C(60) derivatives with two to four malonic acid groups (DMA C(60), TMA C(60) and QMA C(60)) were prepared and the cytotoxicity of these compounds against HeLa cells was determined by MTT. Cell cycle was measured by flow cytometry. The results showed that the cytotoxicity of the malonic acid C(60) derivatives was irradiation- and dose-dependent. The sequence of their photo-induced growth inhibition was DMA C(60)>TMA C(60)>QMA C(60). Hydroxyl radical quencher mannitol (10mM) was not able to prevent cells from the damage induced by irradiated DMA C(60). DMA C(60), together with irradiation, was found to have an ability of inducing a decrease in the number of G(1) cells from 63 to 42\% and a rise in that of G(2)+M cells from 6 to 26\%. These data indicated that the number of malonic acid molecules added to C(60) played an important role in the phototoxicity, and the blockage of cell cycle might be a mechanism of this activity.
This article was published in Toxicol In Vitro
and referenced in Journal of Nanomedicine & Biotherapeutic Discovery