Author(s): Rojanapanthu P, Sarisuta N, Chaturon K, Kraisintu K
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Abstract The physicochemical properties of phosphatidylcholine-cholesterol liposomes containing amphotericin B and prepared by reverse-phase evaporation method were studied. Uniformly dispersed liposomal suspensions were obtained by employing 3:1 ratio (by volume) of diethyl ether to normal saline, 5 min sonication time at 7 degrees C, and evaporation of diethyl ether at 25 degrees C. Microscopic examination showed that the prepared liposomes were spheroids with unilamellar, oligolamellar, or multilamellar structure. The liposomes containing amphotericin B 2.0 mol\% of total lipid led to the highest percentage of drug entrapment. Liposomes with maximum entrapment efficiency were obtained from using 250 micromol of total lipid. The liposomal amphotericin B possessing the highest drug entrapment efficiency (approximately 95\%) with particle size range of 1307-1451 nm was the one composed of 1:1 molar ratio of phosphatidylcholine to cholesterol.
This article was published in Drug Dev Ind Pharm
and referenced in Journal of Clinical & Experimental Ophthalmology