Author(s): Gupta PP
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Abstract Callus cultures were initiated from apical meristems of two commercially cultivated species of cocoyam, Xanthosoma sagittifolium and X. violaceum, on AZ medium supplemented with 2.0 mg 1(-1) 1-naphthaleneacetic acid (NAA). Organ differentiation was achieved from the friable, white callus by using MS medium containing 2.0 mg 1(-1) kinetin (Kn) and 0.2-2.0 mg 1(-1) NAA. This two-step system of plant regeneration could be reduced into one-step in X. violaceum by culturing the explants on MS medium supplemented with 5.0 mg 1(-1) NAA, 100 ml 1(-1) coconut-milk, and 2.0 mg 1(-1) Kn, where compact, green callus formation and organogensis occurred within 8 weeks. The regenerated plants were free of dasheen mosaic virus disease. In X. sagittifolium, various phenotypic variation was noted, such as shape, size and total number of leaves and corms. These alterations may be used to improve cocoyam production.
This article was published in Plant Cell Rep
and referenced in Advancements in Genetic Engineering