alexa Plant Regeneration from Pea Leaflets Cultured in vitro and Genetic Stability of Regenerants.


Journal of Addiction Research & Therapy

Author(s): Rubluo A, Kartha KK, Mroginski LA, Dyck J

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Abstract Bud and shoot formation and regeneration of complete plants have been achieved with immature and mature leaflets, at various frequency, under the influence of a range of cytokinin and auxin combinations. Immature leaflets, 0.9 to 1.8 mm in length, produced shoots in 26 to 38\% explants on a medium containing 10.0 μM BA and NAA, IBA or IAA. A reduction in the frequency of bud and shoot regeneration to 7\% in the presence of 10.0 μM each of BA + NAA was observed with mature leaflets. In the differentiation of buds and shoots, no stimulating effect of GA(3) has been observed. Combinations of cytokinins with picloram and 2,4-D suppressed organogenesis. Plant regeneration was influenced by genotype and temperatures. Seed-derived progeny of regenerants contained the normal diploid number of chromosomes (2n = 14). Analysis of four isozymes, esterase, GDH, 6-PGD, and LAP, as well as nine genetically defined morphological characters indicated retention of genetic stability in the progeny of tisssue culture propagules. Copyright © 1984 Gustav Fischer Verlag, Stuttgart. Published by Elsevier GmbH.. All rights reserved. This article was published in J Plant Physiol and referenced in Journal of Addiction Research & Therapy

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