alexa Plasmid-mediated dimethoate degradation in Pseudomonas aeruginosa MCMB-427.
Agri and Aquaculture

Agri and Aquaculture

Journal of Aquaculture Research & Development

Author(s): Deshpande NM, Dhakephalkar PK, Kanekar PP

Abstract Share this page

Abstract AIMS: To investigate the genetics of dimethoate degradation in Pseudomonas aeruginosa MCMB-427. METHODS AND RESULTS: Pseudomonas aeruginosa MCMB-427 demonstrated the ability to degrade dimethoate, a synthetic organophosphate insecticide. Total DNA preparation of MCMB-427 revealed the presence of a 6.6 kbp plasmid (designated as pDMD427). Escherichia coli NovaBlue transformed with plasmid pDMD427 subsequently acquired the ability to degrade dimethoate. Curing of the plasmid by plumbagin or ethidium bromide resulted in the loss of ability of MCMB-427 to degrade dimethoate. Plasmid pDMD427 was stable in MCMB-427 over 20 passages without selection. Genes encoding resistance to norfloxacin and cobalt were also located on plasmid pDMD427. CONCLUSION: The ability of Ps. aeruginosa MCMB-427 to degrade dimethoate is plasmid-mediated and transferable to other strains. SIGNIFICANCE AND IMPACT OF THE STUDY: As far as is known, this is the first report of plasmid-mediated dimethoate biodegradation. This study contributes significantly towards an understanding of the genetics of bacterial dimethoate degradation.
This article was published in Lett Appl Microbiol and referenced in Journal of Aquaculture Research & Development

Relevant Expert PPTs

Relevant Speaker PPTs

Recommended Conferences

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version