alexa Poly(3-hydroxybutyrate) synthesis in fed-batch culture of Ralstonia eutropha with phosphate limitation under different glucose concentrations.
Microbiology

Microbiology

Journal of Microbial & Biochemical Technology

Author(s): Shang L, Jiang M, Chang HN

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Abstract Poly(3-hydroxybutyrate) (PHB) was produced by fed-batch cultures of Ralstonia eutropha with phosphate limitation under different glucose concentrations. When glucose was kept at 2.5 g l(-1), cell growth and PHB synthesis were limited due to the shortage of carbon source but a higher PHB content occurred in the cell-growth stage. This shows that a low glucose concentration is favorable for PHB accumulation in R. eutropha. PHB obtained with glucose at 9 g l(-1) is 1.6 times that obtained with 40 g l(-1). When glucose was in the range of 9 to 40 g l(-1), PHB concentration and productivity decreased significantly with the increase of glucose concentration. The highest PHB productivity was obtained with glucose at 9 g l(-1).
This article was published in Biotechnol Lett and referenced in Journal of Microbial & Biochemical Technology

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