Author(s): Xu Y, Du Y, Huang R, Gao L
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Abstract N-(2-hydroxyl) propyl-3-trimethyl ammonium chitosan chloride (HTCC) is water-soluble derivative of chitosan (CS), synthesized by the reaction between glycidyl-trimethyl-ammonium chloride and CS. HTCC nanoparticles have been formed based on ionic gelation process of HTCC and sodium tripolyphosphate (TPP). Bovine serum albumin (BSA), as a model protein drug, was incorporated into the HTCC nanoparticles. HTCC nanoparticles were 110-180 nm in size, and their encapsulation efficiency was up to 90\%. In vitro release studies showed a burst effect and a slow and continuous release followed. Encapsulation efficiency was obviously increased with increase of initial BSA concentration. Increasing TPP concentration from 0.5 to 0.7 mg/ml promoted encapsulation efficiency from 46.7\% to 90\%, and delayed release. As for modified HTCC nanoparticles, adding polyethylene glycol (PEG) or sodium alginate obviously decreased the burst effect of BSA from 42\% to 18\%. Encapsulation efficiency was significantly reduced from 47.6\% to 2\% with increase of PEG from 1.0 to 20.0 mg/ml. Encapsulation efficiency was increased from 14.5\% to 25.4\% with increase of alginate from 0.3 to 1.0 mg/ml.
This article was published in Biomaterials
and referenced in Journal of Molecular and Genetic Medicine