Author(s): Gupta V, Datta P, Chander J
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Abstract OBJECTIVE: Gram-negative bacteria with acquired metallo-beta lactamases production have been increasingly reported in some countries, necessitating their detection. We determined the occurrence of acquired metallo-beta lactamases (MBL) producing bacteria in our teaching hospital. METHOD: The method employed was a rapid technique using a disk of imipenem plus 750 microg of EDTA, on Mueller-Hinton plate which was supplemented with 70 microg/ml of zinc sulfate, to differentiate MBL producing Pseudomonads and Acinetobacter. RESULT: Out of 200 isolates tested, 7.5\% of our Pseudomonads and Acinetobacter were shown to be MBL producers. CONCLUSIONS: Though MBL prevalence is still low in our hospital set up, screening for MBL among Pseudomonas and Acinetobacter isolates should be routinely done in laboratory.
This article was published in J Infect
and referenced in Journal of Proteomics & Bioinformatics