Author(s): Pattinson HA
A single simple laboratory test for the detection of sperm surface antibodies is described. Spermatozoa from 300 men who were investigated for infertility were tested for sperm surface antibodies using the Immunobead test (IBT). IBTs for IgG, IgA, and IgM were performed on each sample as well as an IBT using an Immunobead with activity against both light and heavy immunoglobulin chains and therefore capable of detecting any of the three isotypes ("GAM-IBT"). An excellent correspondence was found between GAM-IBT-positive samples and those positive by one or more of the isotype-specific Immunobeads. In 29 of 32 positive tests, the GAM bead binding was within 5% of the most positive isotype-specific bead's binding. There were no false-positive or false-negative tests comparing the GAM-IBT with the isotype-specific IBTs.