Author(s): Berger P, Adkambi T, Mallet MN, Drancourt M
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Abstract We report a bacterial isolate (Marseille isolate) recovered from the blood of a patient hospitalized in an intensive care unit, presenting with severe trauma, fever and mechanical ventilation. Colonies appeared at 37 degrees C on blood agar after 72 h incubation. This isolate was a strictly anaerobic, Gram-negative rod phenotypically related to other Prevotella species described to date: non-motile, catalase-negative, oxidase-positive, non-glucose fermenting, resistant to vancomycin and susceptible to kanamycin. Cells exhibited a trilamellar membrane under electron microscopy. The fatty acid methyl ester profile was marginally related to that of Clostridium botulinum group A (distance: 26.27\%) and Bifidobacterium bifidum GC subgroup B (distance: 26.38\%). 16S rRNA gene sequence similarity was 90.0\% with that of Prevotella oris and 89.1\% with that of Prevotella melaninogenica. Partial rpoB gene sequence similarity was 84.5 and 86.4\% with P. oris and P. melaninogenica, respectively. According to current standards, phenotypic traits, 16S rRNA and rpoB gene sequence analyses indicated that the Marseille isolate belonged to a previously unrecognized species of the genus Prevotella, and we propose classifying it in the new taxon "Prevotella massiliensis" sp. nov.
This article was published in Res Microbiol
and referenced in Journal of Clinical Case Reports