alexa Primary metabolites and flavonoid constituents of Isatis microcarpa J. Gay ex Boiss.
Chemical Engineering

Chemical Engineering

Journal of Chromatography & Separation Techniques

Author(s): Shalabia Shahat Emam, Abd ElMoaty, Mohamed S Abd El

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Phytochemical studies on Isatis microcarpa J. Gay ex Boiss (Family- Cruciferae ) revealed that the plant water content was high at March, while the total ash, acid soluble and insoluble ash, beside the crude fibers of Isatis microcarpa were high at May. Meanwhile the total carbohydrates, nitrogen contents and lipids contents reach its high values at April. Preliminary phytochemical screening indicated that the plant contains coumarins, tannins, sterols and/or terpens, flavonoids, glycosides and/or carbohydrates and resins. The plant contained five free sugars, eight combined sugars, ten free amino acids and fifteen protein amino acids. While GLC of Saponifiable matter revealed the presence of seven saturated fatty acids and two unsaturated fatty acids. Chromatographic investigation of flavonoids constituents of Isatis microcarpa revealed the presence of eight flavonoids ( apigenin-7-O-rutinoside, apigenin-7-O-glucoside, luteolin , luteolin -7-O-glucoside , kaempferol, kaempferol-7-O-glucoside, kaempferol-3- O-glucoside and naringenin). These compounds were identified through R f values, acid hydrolysis, enzymatic hydrolysis, UV spectral data, 1 H-NMR analysis and mass spectral data. The microbiological activities (six bacterial and fungal strains) of the plant extracts on microorganisms revealed that the ethyl alcohol (96% and 70%) and water extracts showed the best effect on all the tested bacteria and fungi

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This article was published in Journal of Natural Products and referenced in Journal of Chromatography & Separation Techniques

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