alexa Principles of affinity-based biosensors.
Engineering

Engineering

Journal of Biosensors & Bioelectronics

Author(s): Rogers KR

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Abstract Despite the amount of resources that have been invested by national and international academic, government, and commercial sectors to develop affinity-based biosensor products, little obvious success has been realized through commercialization of these devices for specific applications (such as the enzyme biosensors for blood glucose analysis). Nevertheless, the fastest growing area in the biosensors research literature continues to involve advances in affinity-based biosensors and biosensor-related methods. Numerous biosensor techniques have been reported that allow researchers to better study the kinetics, structure, and (solid/liquid) interface phenomena associated with protein-ligand binding interactions. In addition, potential application areas for which affinity-based biosensor techniques show promise include clinical/diagnostics, food processing, military/antiterrorism, and environmental monitoring. The design and structural features of these devices--composed of a biological affinity element interfaced to a signal transducer--primarily determine their operational characteristics. This paper, although not intended as a comprehensive review, will outline the principles of affinity biosensors with respect to potential application areas. This article was published in Mol Biotechnol and referenced in Journal of Biosensors & Bioelectronics

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