alexa Production of a recombinant antimicrobial peptide in transgenic plants using a modified VMA intein expression system.
Medicine

Medicine

Advanced Techniques in Biology & Medicine

Author(s): Morassutti C, De Amicis F, Skerlavaj B, Zanetti M, Marchetti S

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Abstract Tobacco plants were engineered to express SMAP-29, a mammalian antimicrobial peptide of innate immunity, as fusion protein with modified vacuolar membrane ATPase intein. The peptide was purified taking advantage of the intein-mediated self-cleaving mechanism. SMAP-29 was immunologically detected in the chromatographic eluate and appeared tightly bound to copurified plant proteins. Electrophoretic separation under disaggregating conditions indicated that the recombinant peptide was cleaved off by intein at the expected site and an overlay gel assay demonstrated that the peptide retained antimicrobial activity. These results indicate that a modified intein expression system can be used to produce pharmaceutical peptides in transgenic plants.
This article was published in FEBS Lett and referenced in Advanced Techniques in Biology & Medicine

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