alexa Production of chlorothalonil hydrolytic dehalogenase from agro-industrial wastewater and its application in raw food cleaning.


Irrigation & Drainage Systems Engineering

Author(s): Qin He XX, Zhang F, Tai YK, Luo YF, He J,

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Abstract BACKGROUND: To reduce the fermentation cost for industrialization of chlorothalonil hydrolytic dehalogenase (Chd), agro-industrial wastewater including molasses, corn steep liquor (CSL) and fermentation wastewater were used to substitute the expensive carbon and nitrogen sources and fresh water for lab preparation. RESULTS: The results showed that molasses and CSL could replace 5\% carbon source and 100\% organic nitrogen source to maintain the same fermentation level. Refermentation from raffinate of ultra-filtered fermentation wastewater could achieve 61.03\% of initial Chd activity and reach 96.39\% activity when cultured in mixture of raffinate and 50\% of original medium constituent. Typical raw foods were chosen to evaluate the chlorothalonil removal ability of Chd. After Chd treatment for 2 h at room temperature, 97.40\% and 75.55\% of the 30 mg kg-1 chlorothalonil on cherry tomato and strawberry were reduced respectively, and 60.29\% of the 50 mg kg-1 chlorothalonil on Chinese cabbage was removed. Furthermore, the residual enzyme activity remained 78 ~ 82\% after treatment, suggesting its potential for reuse. CONCLUSION: This study proved the cost-feasibility of large-scale production of Chd from agro-industrial wastewater and demonstrated the potential of Chd in raw food cleaning. This article is protected by copyright. All rights reserved. This article was published in J Sci Food Agric and referenced in Irrigation & Drainage Systems Engineering

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