alexa Production of monoclonal antibodies and enzyme immunoassay for typical adenylate cyclase activator, forskolin.
Pharmaceutical Sciences

Pharmaceutical Sciences

Pharmaceutica Analytica Acta

Author(s): Masato Ishiyama, Hiroki Murakami, Hideo Shinohara, Yukihiro Shoyama

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The ratio of hapten and bovine serum albumin in an antigen conjugate was determined by matrix-assisted laser desorption/ionization mass spectrometry. A hybridoma secreting monoclonal antibodies against solamargine was produced by fusing splenocytes immunized with a solamargine-bovine serum albumin conjugate with HAT-sensitive mouse myeloma cell line, P3-X63-Ag8-653. Extensive cross-reaction of anti-solamargine antibodies against solasonine appeared. Aglycone of solamargine, solasodine cross-reacted with anti-solamargine antibodies resulting in a 43.8% cross-reaction. Insignificant cross-reaction appeared with tomatine (2.06%). The full measuring range of the assay extends from 57.5 pmol ml−1 to 11.5 nmol ml−1 of solamargine.

This article was published in Cytotechnology and referenced in Pharmaceutica Analytica Acta

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