Author(s): Day JB, Mantle PG, Shaw BI
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Abstract A spectrofluorometric assay for the estimation of the tremorgenic mycotoxin verruculogen in crude mycelial extract has been devised and used to determine concentrations as low as 0.2 microgram ml-1. Verruculogen production by Penicillium estinogenum has been extended from surface culture to submerged culture in 60 1 stirred fermenters, in which the maximum cell-associated mycotoxin yield [5 mg (100 ml culture)-1] was obtained within 7 d. It was found necessary to supplement the medium (Czapek Dox broth plus 0.5\% yeast extract) with calcium chloride (2\%) to induce profuse sporulation (2 X 10(7) conidia ml-1).
This article was published in J Gen Microbiol
and referenced in Journal of Molecular and Genetic Medicine